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Capillary Electrophoresis-Mass Spectrometry

Therapeutic Protein Characterization

Lichao Zhang (Hrsg.), James Q. Xia (Hrsg.)

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Springer International Publishing img Link Publisher

Naturwissenschaften, Medizin, Informatik, Technik / Biochemie, Biophysik

Beschreibung

This book covers the latest developments in capillary electrophoresis-mass spectrometry for the analysis of therapeutic proteins. The application of capillary electrophoresis-mass spectrometry (CE-MS) coupling technology in the analysis of recombinant therapeutic proteins is detailed thoroughly. Specific topics include recent developments in coupling capillary electrophoresis with mass spectrometry for the quality control of monoclonal antibody therapeutics, top-down analysis of monoclonal antibody using the CE-MS platform, and detection of host cell protein impurities. Comprehensive characterization of antibody-drug conjugates (ADCs) by coupling capillary electrophoresis with mass spectrometry is also covered. This is an ideal book for scientists in the life science and biopharmaceutical industry who are working on characterizing the PTMs of monoclonal antibodies, as well as graduate students and researchers in the separation science and biological mass spectrometry fields.

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Schlagwörter

characterization of antibody-drug conjugates, cIEF-MS technology, PK study of biologics analysis, combining LC-MS with CE-MS workflow, protein sequence coverage, posttranslational modifications, PTMs, CE-MS, capillary electrophoresis-mass spectrometry, Monoclonal antibody, recombinant therapeutic proteins, ADCs, Protein glycosylation profiling, peptide mapping of monoclonal antibody, mass spectrometry