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Rapid Cell Magnetisation Using Cationised Magnetoferritin

Sara Correia Carreira

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Springer International Publishing img Link Publisher

Naturwissenschaften, Medizin, Informatik, Technik / Biochemie, Biophysik

Beschreibung

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.

 

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Schlagwörter

Magnetic Nanoparticles, Magnetic Cell Separation, Magnetoferritin, Magnetic capture of pathogens, Stem Cell Magnetization, Bacteria Magnetisation, Protein Modification, Magnetic labelling of biological cells, Bacteria Isolation