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Investigation of Nanoscopic Dynamics and Potentials by Interferometric Scattering Microscopy

Jaime Ortega Arroyo

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Springer International Publishing img Link Publisher

Naturwissenschaften, Medizin, Informatik, Technik / Physikalische Chemie

Beschreibung

This thesis offers a unique guide to the development and application of ultrasensitive optical microscopy based on light scattering. Divided into eight chapters, it covers an impressive range of scientific fields, from basic optical physics to molecular biology and synthetic organic chemistry. Especially the detailed information provided on how to design, build and implement an interferometric scattering microscope, as well as the descriptions of all instrumentation, hardware interfacing and image processing necessary to achieve the highest levels of performance, will be of interest to researchers now entering the field.

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Schlagwörter

Myosin 5a Dynamics, Interferometric Scattering Microscopy (iSCAT), Physical Autocatalysis, Single-molecule Approaches to Optical Microscopy, Membrane Biophysics, Ultrasensitive Optical Microscopy, High Speed Single-particle Tracking, Label-free Imaging, Label-free Single-protein Detection