Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging

Anna Siri Luthman

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Springer International Publishing img Link Publisher

Naturwissenschaften, Medizin, Informatik, Technik / Mechanik, Akustik

Beschreibung

This book describes the design, development, characterisation and application of two novel fluorescence imaging instruments based on spectrally resolved detector arrays (SRDAs). The simplest SRDA is the standard colour camera, which integrates a Bayer filter array of red, green and blue colour filters to replicate the colour sensing capability of the human eye. The SRDAs used in this book contain many more colours, ranging from 16 to over 100 colour channels. Using these compact, robust and low-cost detectors for biomedical applications opens new avenues of exploration that were not possible before, in particular, the use of spectral imaging in endoscopy. The work presented shows for the first time that not only can this new type of camera be used for fluorescence imaging, but also that it is able to resolve signals from up to 7 different dyes – a level of multiplexing not previously achieved in tissue with such compact and robust equipment. Furthermore, it reports the application of a bimodal endoscope performing both reflectance and fluorescence imaging using these cameras in an ex vivo pig oesophagus model.

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Schlagwörter

Endoscopy, Hyperspectral Imaging, Camera Calibration, Spectrally Resolving Detector Arrays, In Vivo Imaging, Multispectral Imaging