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Proteomics in Practice

A Guide to Successful Experimental Design

Tom Naven, Reiner Westermeier, Hans-Rudolf Höpker, et al.

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Wiley-VCH img Link Publisher

Naturwissenschaften, Medizin, Informatik, Technik / Genetik, Gentechnik

Beschreibung

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

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Schlagwörter

Genomforschung u. Proteomik, Biochemistry (Chemical Biology), Biotechnology, Chemistry, Biologie / Biochemie, Biophysik, Genomics & Proteomics, Proteomanalyse, Cell & Molecular Biology, Life Sciences, Biochemie u. Chemische Biologie, Biotechnologie i. d. Biowissenschaften, Molekularbiologie, Chemie, Zell- u. Molekularbiologie, Biowissenschaften