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Nano-Bio Probe Design and Its Application for Biochemical Analysis

Bin-Cheng Yin, Min Zhang, Bang-Ce Ye, et al.

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Springer Berlin img Link Publisher

Naturwissenschaften, Medizin, Informatik, Technik / Chemische Technik

Beschreibung

In this volume, Prof. Ye and his coworkers propose and review the concept of nano-bio probe design for biochemical analysis on the basis of their recent published works. A unique biochemical analysis technology based on fluorescence polarization enhanced by nanoparticles is described here with successful applications in environmental monitoring, rapid and sensitive sensing protease activity and high-throughput screening of inhibitors. Furthermore, they introduce a versatile molecular beacon (MB)-like probe for the multiplex sensing of targets such as sequence-specific DNA, protein, metal ions and small molecule compounds based on the self-assembled biomolecule-graphene conjugates. Besides, some colorimetric and luminescence probes utilizing metal nanoparticles for biochemical applications are also presented, such as chiral enantiomer discrimination and separation, environmental monitoring, clinic diagnosis and etc.

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Schlagwörter

colorimetric assay, nano-materials, biochemical engineering, nano-probe, biochemical analysis, biochemical molecules, fluorescence polarization