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Principles of Genomics and Proteomics
Sheikh Mansoor Shafi, Sajad Majeed Zargar, Rakeeb Ahmad Mir, et al.
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Naturwissenschaften, Medizin, Informatik, Technik / Biologie
Beschreibung
Principles of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments. Following an introductory chapter, the book dives into proper research, including genome mapping. Experiments covered in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, and protein resolution methods, including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are also described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction. A final proteome analysis is dedicated to functional analysis. Other chapters cover applications of omics technologies broadly. This book is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods and established researchers will benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments. Provides a comprehensive accounting of the technical aspects of genomic and proteomic analysis and applications in living organisms Includes detailed and standardized coverage of basic research methods in areas covered Presents advanced applications of genomics and proteomics that are highlighted with major significance in medical and agricultural fields
