Recombinant DNA Laboratory Manual, Revised Edition

Judith W. Zyskind, Sanford I. Bernstein

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Elsevier Science img Link Publisher

Naturwissenschaften, Medizin, Informatik, Technik / Naturwissenschaften allgemein

Beschreibung

The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. Comb-bound, three-column, large 9-1/4"e; x 7-1/2"e; format Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts Necessary reagents and equipment are presented in a checklist at the beginning of each protocol Techniques for bacteria are complemented with those for Drosophila Each experiment has been tested in the laboratory by students for five years Features a complete chapter on computers in the molecular biology laboratory Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains

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